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. 2000 Dec 18;192(12):1731–1744. doi: 10.1084/jem.192.12.1731

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© 2000 The Rockefeller University Press

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(A) Subcellular analysis of eNOS as detected by immunoblotting. Membrane-associated (M) and cytosolic (C) eNOS expression. Cytosolic and membrane fractions were prepared from homogenates of each age group and analyzed by immunoblotting. Data are representative of three experiments. HUVECs, human umbilical vein endothelial cells. (B) Activity of total NOS and eNOS in aortas from young and old rats. Activity was measured in homogenates of aortic tissue by determination of the rate of conversion of l-[¹⁴C]arginine to l-[¹⁴C]citrulline. Significance: total NOS, *P < 0.01; eNOS, **P < 0.001.

(A) Subcellular analysis of eNOS as detected by immunoblotting. Membrane-associated (M) and cytosolic (C) eNOS expression. Cytosolic and membrane fractions were prepared from homogenates of each age group and analyzed by immunoblotting. Data are representative of three experiments. HUVECs, human umbilical vein endothelial cells. (B) Activity of total NOS and eNOS in aortas from young and old rats. Activity was measured in homogenates of aortic tissue by determination of the rate of conversion of l-[¹⁴C]arginine to l-[¹⁴C]citrulline. Significance: total NOS, *P < 0.01; eNOS, **P < 0.001.