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. 2000 Dec 18;192(12):1685–1696. doi: 10.1084/jem.192.12.1685

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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MHC class I–restricted cross-presentation is restricted to lymphoid DCs as early as 2 h after priming. Low density cells were isolated from mice 2 or 14 h after priming with OVA-loaded β2m^−/− cells. Myeloid and lymphoid DCs were FACS^® sorted based on their differential CD11b/CD11c expression and tested for their ability to stimulate naive OT-I cells in vitro. The different bars represent titered numbers of DCs in the well. Error bars indicate SEM of triplicate wells. MyDC, myeloid DCs; LyDC, lymphoid DCs; n.t., not tested.