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. 2000 Dec 18;192(12):1719–1730. doi: 10.1084/jem.192.12.1719

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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TCR Vβ CDR3 sequence analysis of CD4^high LDA T cell clones. CD4^highVβ8⁺ cells sorted from the primed LNs at days 3, 6, and 28 after primary immunization and at day 4 after secondary immunization with SWM 110–121 were expanded from limiting dilution cultures. For each selected time point, T cell clones were derived from three independent immunizations. TCR Vβ CDR3 sequence analysis was performed on (A) 47 LDA clones from day 3, (B) 49 LDA clones from day 6, (C) 22 LDA clones from day 28 after primary immunization, and (D) 52 LDA clones from day 4 after secondary immunization. Each clone was tested for antigen specificity in a proliferation assay (Fig. 4, and data not shown). The alignment is based on common use of the Vβ8 segment. Boxes separate germline sequences from N-region additions. Each TCR Dβ and Jβ assignment is displayed next to the corresponding sequence. The predicted amino acid sequence is displayed below each corresponding sequence. Sequences which contain the CDR3 amino acid composition (A/G)WDWx(x) (in bold) are listed as canonical; the other sequences are considered noncanonical. The numbers of clones displaying a particular clonotypic sequence as well as percentages of canonical and noncanonical sequences are listed. *No assignment to either a Dβ1 or a Dβ2 germline sequence could be made.

TCR Vβ CDR3 sequence analysis of CD4^high LDA T cell clones. CD4^highVβ8⁺ cells sorted from the primed LNs at days 3, 6, and 28 after primary immunization and at day 4 after secondary immunization with SWM 110–121 were expanded from limiting dilution cultures. For each selected time point, T cell clones were derived from three independent immunizations. TCR Vβ CDR3 sequence analysis was performed on (A) 47 LDA clones from day 3, (B) 49 LDA clones from day 6, (C) 22 LDA clones from day 28 after primary immunization, and (D) 52 LDA clones from day 4 after secondary immunization. Each clone was tested for antigen specificity in a proliferation assay (Fig. 4, and data not shown). The alignment is based on common use of the Vβ8 segment. Boxes separate germline sequences from N-region additions. Each TCR Dβ and Jβ assignment is displayed next to the corresponding sequence. The predicted amino acid sequence is displayed below each corresponding sequence. Sequences which contain the CDR3 amino acid composition (A/G)WDWx(x) (in bold) are listed as canonical; the other sequences are considered noncanonical. The numbers of clones displaying a particular clonotypic sequence as well as percentages of canonical and noncanonical sequences are listed. *No assignment to either a Dβ1 or a Dβ2 germline sequence could be made.