Table 1.
Somatic Mutations of the CD95 Gene in Naive, GC, and Memory B Cells
| Mutated cells | ||||||
|---|---|---|---|---|---|---|
| B cell subset;Phenotype | Donor | Method | Overall | 5′R | Exon IX | Ig VH |
| Naive; | I | Cloning | 1/21 | ND | 1/21 | 0/17 |
| IgD+IgM+CD27− | II | Single cell | 0/19 | 0/19 | 0/14 | 0/4 |
| III | Single cell | 0/12 | 0/9 | 0/9 | ND | |
| IV | Single cell | 1/11 | 1/8 | 0/7 | 0/1 | |
| V | Single cell | 0/14 | 0/12 | 0/8 | 0/3 | |
| VI | Single cell | 0/14 | ND | 0/14 | ND | |
| VII | Single cell | 0/14 | ND | 0/14 | ND | |
| ΣII–VII | Single cell | 1/84 | 1/48 | 0/66 | 0/8 | |
| GC; | I | Cloning | 4/30 | ND | 4/30 | 13/13 |
| CD38+CD77+ | II | Single cell | 4/25 | 3/25 | 1/9 | 5/5 |
| III | Single cell | 3/15 | 3/14 | 0/5 | 4/4 | |
| IV | Single cell | 2/15 | 1/10 | 1/6 | 4/4 | |
| V | Single cell | 3/14 | 3/11 | 0/2 | 4/4 | |
| VI | Single cell | 0/12 | ND | 0/12 | ND | |
| VII | Single cell | 0/11 | ND | 0/11 | ND | |
| ΣII–VII | Single cell | 12/92 | 10/60 | 2/45 | 17/17 | |
| Anti-CD95–resistant GC; | II | Single cell | 5/5 | 3/4 | 3/5 | ND |
| Annexin V–negative, | III | Single cell | 1/7 | 0/2 | 1/7 | 2/2 |
| CD38+ | IV | Single cell | 2/5 | 0/4 | 2/5 | ND |
| V | Single cell | 1/5 | ND | 1/5 | 2/2 | |
| ΣII–V | Single cell | 9/22 | 3/10 | 7/22 | 4/4 | |
| Memory; | II | Single cell | 1/13 | 1/7 | 0/9 | 4/4 |
| IgD−CD20+CD27+ | III | Single cell | 2/8 | 2/4 | 0/8 | 5/5 |
| IV | Single cell | 0/8 | 0/3 | 0/8 | 4/4 | |
| V | Single cell | 2/8 | 2/3 | 0/8 | 3/3 | |
| ΣII–V | Single cell | 5/37 | 5/17 | 0/33 | 16/16 | |