Figure 1.

Peptide dissociation from wt and DR1_βG86Y. Dissociation kinetics of wt (○) and sDR1_βG86Y (•) complexes. DR1 in complex with FITC-labeled HA_Anchorless was produced and separated. The labeled complexes were dissociated in the presence of 100 times molar excess of relevant unlabeled peptides at 37°C in PBS for various times. The fluorescence of the labeled complex before dissociation (f ₀) is arbitrarily assigned a value of 1.0. The fluorescence of the labeled complex after dissociation for various times is expressed as a percentage of f ₀. The dissociation data are fitted to a single exponential curve that yields t _1/2 of 1.8 ± 0.25 h. The y axis represents arbitrary fluorescence units.