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. 2000 Dec 18;192(12):1785–1796. doi: 10.1084/jem.192.12.1785

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Expression of CD34 and CD45RA on lineage-negative cells reveals four different subpopulations in human fetal liver, fetal bone marrow, fetal thymus, cord blood, and adult peripheral blood samples. Lineage-positive cells (including T, B, and NK cells, and monocytes, granulocytes, and erythrocytes) were depleted using magnetic beads. The enriched cells were analyzed by four-color flow cytometric analysis after staining with an FITC-conjugated cocktail of antibodies against lineage markers (including CD3, CD11c, CD14, CD15, CD16, CD20, and CD57), anti-CD34–APC, anti-CD45RA–Tricolor, and the PE-conjugated antibodies anti-CD4 and anti–IL-3Rα. After electronic gating on FITC-negative cells, four populations of cells (A, B, C, and D) were identified: population A, CD34⁺⁺CD45RA⁻; population B, CD34⁺⁺CD45RA⁺; population C, CD34⁺CD45RA⁺; and population D, CD34⁻CD45RA⁺.