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Generation of IFN-α–producing cells from CD34++ CD45RA− cells with FLT3 ligand or SCF. Sorted CD34++CD45RA− cells were cultured with one of the following cytokines: IL-3, IL-7, SCF, FLT3 ligand (FLT3-L), GM-CSF, or G-CSF. After 11 and 25 d of culture, equal numbers of cells were stimulated with HSV-1 for 24 h and IFN-α (pg/ml) in the supernatants analyzed by ELISA.
