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. 2000 Dec 18;192(12):1785–1796. doi: 10.1084/jem.192.12.1785

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Kinetic analysis on the ability of FLT3 ligand to generate pre-DC2/IPCs from CD34⁺⁺CD45RA⁻ cells. Sorted CD34⁺⁺CD45RA⁻ cells were cultured for a period up till 57 d with FLT3 ligand. Every 5 d, cells were harvested and analyzed. (A) Equal numbers of cells were stimulated with HSV-1 (10 PFU/cell) for 24 h. The amount of IFN-α (pg/ml) produced in the supernatants was measured by ELISA. (B) Phenotypical analysis by flow cytometry after staining with CD11c-FITC, IL-3Rα–PE, and HLA-DR–CyChrome. Shaded histograms show the IL-3Rα expression after electronic gating on HLA-DR⁺CD11c⁻ cells. Open histograms represent staining with an isotype control antibody. (C) Graphical representation of the percentages of IL-3Rα⁺⁺ cells gated in B.