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. 2000 Dec 18;192(12):1809–1818. doi: 10.1084/jem.192.12.1809

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Increased expression of TNF-α during CDE diet feeding. TNF-α levels were detected by ribonuclease protection assay and quantified by densitometry relative to GAPDH. TNF levels increased during the first few days of the CDE diet (A). A peak in TNF-α expression in CDE samples (gray bars) compared with WT (white bars) was seen at 2 wk (B). Repeat experiments show the result illustrated in the representative autoradiograph to be reproducible (bars represent mean ± SEM, n = 6). Analysis of TNF-α mRNA in purified cell types showed expression in oval (OC) and inflammatory (IC) cells but not in hepatocytes (H) (C).

Increased expression of TNF-α during CDE diet feeding. TNF-α levels were detected by ribonuclease protection assay and quantified by densitometry relative to GAPDH. TNF levels increased during the first few days of the CDE diet (A). A peak in TNF-α expression in CDE samples (gray bars) compared with WT (white bars) was seen at 2 wk (B). Repeat experiments show the result illustrated in the representative autoradiograph to be reproducible (bars represent mean ± SEM, n = 6). Analysis of TNF-α mRNA in purified cell types showed expression in oval (OC) and inflammatory (IC) cells but not in hepatocytes (H) (C).