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. 2000 Jun 5;191(11):1819–1828. doi: 10.1084/jem.191.11.1819

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Inhibition of DEVDase activity in apoptotic thymocyte extract using PhiPhiLux™ substrate. An extract of mouse thymocytes exposed to 0.1 μM dexamethasone for 6 h was incubated in fluorometer cuvettes at 37°C with 10 μM PhiPhiLux™ substrate with or without the indicated protease inhibitors. Enzymatic activity was calculated from the rate of increase of fluorescence.