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. 2000 Jun 5;191(11):1819–1828. doi: 10.1084/jem.191.11.1819

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Effect of ZVAD(OMe)-FMK treatment on in situ caspase activation. Thymocytes were treated with 0.1 μM dexamethasone in vitro and analyzed after a total of 6 h incubation. The apoptosis inhibitor ZVAD(OMe)-FMK was added at various times of incubation as indicated, and the caspase activities were assessed by flow cytometry. The results are expressed as ratios of mean fluorescence channel number comparing the activity in the presence of 50 μM Z-VAD(OMe)-FMK with the uninhibited sample for that substrate.