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. 2000 Jun 5;191(11):1895–1904. doi: 10.1084/jem.191.11.1895

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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CD1d1–αGalCer tetramers stained CD1d1-restricted Vα14-Jα281 NKT cells in vivo. (A) Spleen cells from wild-type, CD1d-deficient, or Vα14-Jα281 TCR α chain transgenic mice in a C57BL/6 background were stained with CD1d1–αGalCer tetramers-PE, CD5-Cychrome, and anti–I-A^b–FITC. Dot plots were gated on MHC class II⁻ splenocytes. (B) Splenocytes from normal mice and Vα14-Jα281 TCR α chain transgenics were stained with tetramer-Cychrome, CD5-APC, and anti-CD4 or CD8-FITC. Histograms display the frequency of CD4⁺ and/or CD8⁺ cells among gated CD5⁺ tetramer⁺ splenocytes.