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. 2000 Jun 5;191(11):1895–1904. doi: 10.1084/jem.191.11.1895

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Mouse CD1d1–αGalCer tetramers stain human Vα24/Vβ11 NKT cells. Human PBLs were stimulated with 0.1 μM αGalCer in vitro and stained after 7 d in culture. In this experiment, CD1d1-mediated αGalCer presentation to NKT cells is provided by the PBLs themselves. After centrifugation through Ficoll, the cells were preincubated with anti-TCR antibodies for 1 h before adding tetramer-PE. Note that anti-Vα24 completely blocks tetramer binding.