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. 2000 Jun 5;191(11):1895–1904. doi: 10.1084/jem.191.11.1895

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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CD1d1–αGalCer tetramers do not stain NK cells. Fresh splenocytes (A) or spleen-derived A-LAK cells (B) were stained with CD5-APC, NK1.1-PE, tetramer-Cychrome, and TCR-β–FITC. Less than 1% of NK cells (α/β sTCR⁻NK1.1⁺) were tetramer⁺.