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. 2000 Jun 5;191(11):1905–1920. doi: 10.1084/jem.191.11.1905

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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The effect of RhoGDI on the interaction between N-WASP and VirG in Xenopus egg extracts. (A) Cy2-labeled recombinant N-WASP (at 75 nM) was added to the egg extracts without (a, c, e, and g) or with (b, d, f, and h) RhoGDI (at 400 nM). a and b, DAPI-labeled bacteria; c and d, Cy2-labeled N-WASP; e and f, TMR-actin. The yellow color in the combined image shown in g and h indicates the colocalization between N-WASP (green) and actin (red). The images were observed 10 min after mixing bacteria with extracts. (B) Quantitation of N-WASP intensity, bacterial motility, and F-actin intensity without or with RhoGDI (at 400 nM). White bars, N-WASP intensities; black bars, bacterial speeds; gray bars, F-actin intensities. All activities are normalized to untreated extracts. Error bars represent SEM from three separate experiments. Bar, 10 μm.

The effect of RhoGDI on the interaction between N-WASP and VirG in Xenopus egg extracts. (A) Cy2-labeled recombinant N-WASP (at 75 nM) was added to the egg extracts without (a, c, e, and g) or with (b, d, f, and h) RhoGDI (at 400 nM). a and b, DAPI-labeled bacteria; c and d, Cy2-labeled N-WASP; e and f, TMR-actin. The yellow color in the combined image shown in g and h indicates the colocalization between N-WASP (green) and actin (red). The images were observed 10 min after mixing bacteria with extracts. (B) Quantitation of N-WASP intensity, bacterial motility, and F-actin intensity without or with RhoGDI (at 400 nM). White bars, N-WASP intensities; black bars, bacterial speeds; gray bars, F-actin intensities. All activities are normalized to untreated extracts. Error bars represent SEM from three separate experiments. Bar, 10 μm.