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. 2000 Jun 5;191(11):1905–1920. doi: 10.1084/jem.191.11.1905

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© 2000 The Rockefeller University Press

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In vitro reconstitution of actin assembly on E. coli (VirG) and Listeria surface. (A) E. coli expressing VirG were incubated in F buffer containing 0.4 μM N-WASP, 70 nM Arp2/3 complex, and 1 μM TMR-actin, with or without 0.4 μM Cdc42-G12V (GTPγS) expressed by baculovirus. (B) Listeria were incubated in F buffer containing 70 nM Arp2/3 complex and 1 μM TMR-actin: a, d, and g, DAPI-labeled bacteria; b, e, and h, TMR-actin; c, f, and i, merged images. Insets show magnified images. Bar, 10 μm.