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. 2000 Jun 5;191(11):1987–1998. doi: 10.1084/jem.191.11.1987

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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CTLA-4 ligation permits HIV-1 infection of CD3/28 bead–stimulated cells. CD4 T cells were stimulated as described in the legend to Fig. 1 for 3 d and infected with the R5 isolates as described in Materials and Methods. Samples were taken at t = 0, 2, 72, and 144 h after infection and cell pellets were analyzed for gag DNA by a quantitative PCR assay using liquid hybridization. p24 (in ng/ml) was measured in culture supernatants at 144 h after infection. Data shown are representative of three experiments.