Table 1.

Level of λ1 Transcripts in scid Fetal Liver and JHT scid Bone Marrow Relative to wt Controls

Genotype	Fetal Liver	Bone Marrow
+/+	1.0	1.0
s/s	1.2, 0.52	–
	(0.76, 0.83)	–
JHT s/s	–	0.001
JHT +/+	–	0.024

PCR-amplified (23 cycles) λ1 transcripts, and also Vλ1–Jλ1 rearrangements in the case of fetal liver, were gel electrophoresed, blotted, and hybridized with a λ1-specific probe (see Materials and Methods). The values shown correspond to the amount of λ1 hybridizing signal normalized against the internal control (β2M) and the reference control, fetal liver or bone marrow of wt (+/+) mice. Thus, for example, the ratio of λ1/β2M hybridizing signal in JHT (JHT +/+) bone marrow divided by the λ1/β2M hybridizing signal in the reference control equaled 0.024. Two values are shown for scid fetal liver. These correspond to the ratios obtained for day 13 and 14 samples, respectively. The two values in parentheses correspond to the ratios obtained for Vλ1–Jλ1 rearrangement in genomic DNA from day 13 and 14 scid fetal liver, respectively.