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. 2008 Feb 6;3(2):e1542. doi: 10.1371/journal.pone.0001542

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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A) Orthologs were predicted between consecutively greater numbers of bacteria, beginning with all pairwise combinations and ending with all of the 17 bacteria. Clustered results reveal a core genome of 144 genes and more exclusive orthologs between bacteria of the same species. B) Observed protein orthologs measured using liquid chromatography tandem mass spectrometry were included and given greater weight than predicted orthologs only. Clustering resulted in improved agreement with phylogenetic predictions. 105 of the 144 core genes were verified by protein observations, which represent the core proteome for the set of bacteria.