Figure 2.

The effect of VAMP knockdowns on insulin-stimulated myc-GLUT4-GFP reporter translocation. Fully differentiated 3T3L1 adipocytes were cotransfected with a random siRNA or VAMP-specific siRNA plus the myc-GLUT4-GFP reporter cDNA. 72 h later, the cells were either left untreated or stimulated with 100 nM insulin for 30 min. The cells were fixed and subjected to confocal fluorescent microscopy for the localization of myc-GLUT4-GFP. These data represent the analysis of 15 cells/experiment from three to five independent experiments. Statistical analysis was performed using a one-way ANOVA followed by the Student-Newman-Keuls multiple range test. Identical letters indicate bars that are not statistically different from each other (P < 0.05). Error bars represent SD.