Figure 6.

The effects of VAMP knockdowns on the sorting of newly synthesized GLUT4. (A) Fully differentiated 3T3L1 adipocytes were transfected with a random siRNA or VAMP-specific siRNA. (B) Fully differentiated adipocytes were cotransfected with a random siRNA or VAMP4-specific siRNA in combination with the indicated VAMP-specific siRNA. (A and B) 60 h later, the cells were transfected with the myc-GLUT4-GFP reporter construct. 12–16 h later, the cells were left untreated or stimulated with 100 nM insulin for 30 min. The cells were fixed and subjected to confocal fluorescent microscopy for the localization of myc-GLUT4-GFP. These data represent the analysis of 15 cells/experiment from three independent experiments. Statistical analysis was performed using a one-way ANOVA followed by the Student-Newman-Keuls multiple range test. Identical letters indicate bars that are not statistically different from each other (P < 0.05). Error bars represent SD.