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. 2008 Jan 28;180(2):389–402. doi: 10.1083/jcb.200707031

Figure 9.

Figure 9.

VPS4B(E235Q)-GFP forms large corral-like rings on the plasma membrane of cells expressing only endogenous ESCRT-III proteins. (top) Overview of plasma membrane from a cell expressing only VPS4B(E235Q)-GFP. A GFP tag on VPS4 was used to selectively immunodecorate these particles with 18 nm gold. (bottom) Selected higher magnification views of rings formed by VPS4B(E235Q)-GFP. Bars: (top) 500 nm; (bottom) 100 nm.