Table II. Summary of gene expression and gene positioning data.
| Day 0→day 20
|
Day 0→Q
|
Q→day 20
|
Day 0→day 20E
|
Day 20→day 20E
|
Q→day 20E
|
|||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Posn | Exp | Posn | Exp | Posn | Exp | Posn | Exp | Posn | Exp, | Posn | Exp | |
| AKT1 | In | NSD | In | ↓ | In | ↑ | In | NSD | P | NSD | NSD | NSD |
| BCL2 | NSD | ↓ | NSD | ↓ | P | ↑ | P | ↓ | P | NSD | P | NSD |
| CCND1 | In | ↓ | NSD | ↓ | In | ↑ | In | ↓ | NSD | NSD | In | ↑ |
| ERBB2 | In | NSD | In | ↑ | In | NSD | In | ↑ | In | NSD | In | ↑ |
| ERBB2IP | NSD | ↑ | NSD | ↑ | In | NSD | NSD | ↑ | NSD | NSD | In | NSD |
| FGFR1 | NSD | NSD | P | NSD | In | ↓ | P | NSD | NSD | ↑ | In | NSD |
| MMP1 | In | ↑ | NSD | NSD | In | ↑ | In | ↑ | NSD | ↑ | In | ↑ |
| TP53 | NSD | ↑ | NSD | NSD | NSD | ↑ | In | NSD | NSD | ↓ | In | NSD |
| PTEN | In | ↑ | In | ↑ | In | NSD | In | ↑ | NSD | ↓ | In | ↓ |
| TGFB3 | Altereda | NSD | Altereda | NSD | NSD | NSD | Altereda | NSD | NSD | NSD | NSD | NSD |
| VEGF | In | ↑ | P | NSD | In | NSD | NSD | ↑ | P | NSD | In | ↑ |
Heading row shows growth conditions being compared. Changes in gene positions (Posn) and in gene expression (Exp) between growth conditions are shown.
Day 0, proliferating 2D cultures; Day 20, control cultures grown in 3D culture for 20 d; Day 20E, ErbB2-activated 3D cultures; In, internal movement; NSD, no significant difference between the growth conditions; P, peripheral movement; Q, quiescent 2D cultures; ↑, up-regulation; ↓, down-regulation.
a
TGFB3 shifts from two subpopulations at day 0 to a single population distribution pattern in all other growth conditions.