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FAK binding directs p190RhoGEF localization to FAs upon FN plating. FAK+/+ MEFs stably expressing GFP or the indicated GFP-p190RhoGEF or GFP-GEFH1 constructs were plated onto 1-μg/ml-FN–coated coverslips for 1 h, fixed, and then stained with antibodies to paxillin. Shown is the cellular GFP (left) or paxillin (middle) distribution and the merged images (right). Bar, 30 μm.
