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. 2008 Jan 14;180(1):159–172. doi: 10.1083/jcb.200707136

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Copyright © 2008, The Rockefeller University Press

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Figure 2. — Hexosaminidase secretion is increased in cells when STX10, STX16, or Vti1a function is compromised. (A) HeLa cells grown in 6-cm dishes were either mock transfected (control) or transfected with the indicated myc-tagged cytosolic SNARE and incubated in fresh media for 8 hto collect secreted hexosaminidase. (B) Immunoblot of overexpressed SNARE proteins from the cells analyzed in A and a portion of the Ponceau S–stained filter as a loading reference. Proteins were detected with anti-myc tag antibody. (C) Hexosaminidase secretion from control or STX10-depleted HeLa cells. The top panel shows a Ponceau S–stained immunoblot membrane (top bar) of HeLa cell lysate reacted with antibodies to monitor STX10 depletion. Numbers at the right in B and C indicate M _r in kilodaltons. (A and C) Values are means from at least three independent experiments performed in triplicate; error bars represent SD.