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. 2008 Jan 14;180(1):159–172. doi: 10.1083/jcb.200707136

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Copyright © 2008, The Rockefeller University Press

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Figure 4. — Depletion of STX10 leads to CI-MPR dispersal to sorting nexin-2–positive structures but does not disrupt Golgin 97 localization. (A) Summation of confocal z sections of HeLa cells treated with STX10 siRNA and double labeled with rabbit anti-STX10 and mouse anti–CI-MPR (top) or rabbit anti-STX10 and mouse anti–Golgin 97 (bottom). Arrows point to cells depleted of STX10. Bar, 10 μm. (B) Quantitation of CI-MPR dispersal in cells depleted of STX10. Peripheral CI-MPR–positive vesicles were determined as described in Materials and methods. The mean number of vesicles from three separate cells normalized for cell area is shown. Error bars represent SD. (C) Deconvolution microscope image showing colocalization of endogenous CI-MPR (stained red with mouse antibody) and sorting nexin-2 (stained green with rabbit antibody) in the periphery of a HeLa cell depleted of STX10. Bar, 5 μm. (D) Venn diagrams representing the quantitation of colocalization between sorting nexin-2 and EEA1 or sorting nexin-2 and CI-MPR in nonperinuclear regions of control and STX10-depleted HeLa cells. Vesicles were from nine (control) or seven (STX10 depleted) separate cells from two independent experiments. Venn diagrams are correctly scaled for percentage overlap to permit the direct comparison of antigens in each vesicle population.