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. 2008 Jan 14;180(1):159–172. doi: 10.1083/jcb.200707136

Figure 8.

Figure 8.

Rab6 competes with STX16 for GCC185 binding. (A) Purified, GCC185–C-110 and GST-soluble STX16 were incubated together, either alone or with Rab6 preloaded with GDP or GTPγS. GST-STX16 was recoveredwith glutathione–Sepharose beads; the bound GCC185–C-110 was analyzed by SDS-PAGE, and the immunoblot was probed with anti-GCC185 antibody. Immunoblots were quantified; the amount of GCC185–C-110 binding to STX16 relative to binding in the absence ofRab protein (control) from two independent experiments is shown. Error bars represent SD. (B) Equal amounts of purified GCC185–C-110 wild type or GCC185–C-110 IL/AA were incubated with GST-tagged soluble STX16. GST-STX16 was recovered with glutathione–Sepharose beads, and the bound proteins were analyzed by SDS-PAGE and immunoblotting with rabbit anti-GCC185 (top) to detect C-110or with rabbit anti-GST (bottom) to detect STX16. Numbers at the left indicate M r in kilodaltons.