Figure 8.

Components of the gene expression machinery are recruited to the lamina-targeted locus upon gene activation. Recruitment of YFP–RNA pol II (A) or YFP-SF2/ASF (B) to targeted and activated locus at the lamina. Targeting cells were transiently transfected with pTet-On and YFP–RNA pol II or YFP-SF2/ASF (green), induced for 12 h with cumate to express the targeting fusion protein (red, arrows), and then incubated in the absence of Dox (A [a–d] or B [a–d]) or presence of Dox (A [e–h] or B [e–h]) for 5 h. In Dox (−) condition, YFP–RNA pol II or YFP-SF2/ASF was not recruited (A [b and d] or B [b and d], arrows) and CFP-SKL protein was not observed (A [c and d] or B [c and d]). In Dox (+) condition, YFP–RNA pol II and YFP-SF2/ASF were recruited (A [f] or B [f], arrows) and localized with the locus (A [h] or B [h], arrows) and CFP-SKL protein was observed at cytoplasmic peroxisomes (A [g and h] or B [g and h]). Bars, 5 μm.