Table III. Spectrum of mutations observed in Can^r colonies from day 7.

Clone	Mutation type			Position from ATG	Sequence
Wild typea
1	Base substitution	C→T	Proline-leucine	656	TGTTCCCTGTC
2	Base substitution	T→G	Asparagine-lysine	1173	AAATTCAAATA
	Insertion	G		1710	GAGGC-G-AATTGT
3	Duplication		248bp	184–431
4	Base substitution	G→C	Alanine-proline	709	TTTTAGCCATTA
	Deletion	TG	Isoleucine-arginineframeshift	1098–1099	TTGCTATTGAGAA
5	Base substitution	G→T	Glycine-valine	353	ACGCCGGCCCAG
	Insertion	A		1341	CAAA-A-GTTTTCG
6	Base substitution	C→T	Proline-serine	937	GCTGCAAACCCCA
7	no PCR
8	Deletion	AT	Frameshift	1129–1130	TGTCACATATCTT
	Insertion	T	Frameshift (T3-T4)	1086	CCCTTT-T-ATTATT
9	Base substitution	G→T	Tryptophan-cysteine	531	GGTTTTCTTGGCA
	Deletion	A	Frame-shift (A3-A2)	663	CAAATATTACGGT
	Insertion	T	Frameshift (T3-T4)	1086	CCCTTT-T-ATTATT
10	Base substitution	G→A	Glutamic acid-lysine	679	GAATTCGAGTTCT
	Base substitution	G→T	Valine-phenylalanine	907	GAACTATTTGGTA
	Deletion	A	Frameshift	1217	TATCAAAGAACAC
sgs1Δ b
1	Base substitution	C→A	Tyrosine-STOP	591	GGACGTACAAAG
	Deletion	A	Frameshift (A6-A5)	969	TCAAAAAAGTTGC
2	Deletion	T	Frameshift	1756	GAATGTTGTAGC
3	partially sequenced
4	no PCR
5	Base substitution	T→C	Valine-alanine	659	GTTCCCTGTCAAA
6	no PCR
7	partially sequenced
8	no PCR
9	Base substitution	T→C	Tryptophan-arginine	529	GGTTTTCTTGGGC
10	Base substitution	C→A	Alanine-aspartic acid	1166	ATTTCTGCCGCAA
sch9Δsgs1Δ c
1	Insertion	CT	Frameshift	1006–1007	GCTCTCT-CT-ATT
2	Base substitution	G→A	Glycine-serine	670	ATTACGGTGAATT
3	Base substitution	G→T	Glutamic acid-STOP	184	TGGCGAGGATAC
4	partially sequenced
5	no PCR
6	Base substitution	A→C	Tyrosine-serine	1184	TATTTACGTTGGT
7	Base substitution	G→T	Glycine-valine	911	TAGTTGGTATCAC
8	Base substitution	G→A	Glycine-serine	922	ACTGCTGGTGAAG
9	Base substitution	C→A	Phenylalan-leucine	1193	ACGTTGGTTCCTA
10	Deletion	T	Frameshift leucine-phenyalan	543	AATCACTTTTGCC
	Base substitution	G→C	No change	544	AATCACTTTTGCC

Base substitutions: bold, insertions; underline, deletions.

^aTotal clones: 10 (no PCR, 1/10; sequenced, 9/10).

^bTotal clones: 10 (no PCR, 3/10; partially sequenced, 2/10; sequenced, 5/10).

^cTotal clones: 10 (no PCR, 1/10; partially sequenced, 1/10; sequenced, 8/10).