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. 2008 Jan 14;180(1):101–112. doi: 10.1083/jcb.200706072

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Copyright © 2008, The Rockefeller University Press

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Figure 5. — Loss of PHB provokes an antiangiogenic phenotype by influencing the cytoskeletal balance in vitro. (A) Actin stress fiber formation (stained with phalloidin) was examined via indirect immunofluorescence analysis using control (upper left) and PHB1 knockdown (top right and bottom) cells stimulated with VEGF (all) and PEG-catalase (bottom) and stained with rhodamin-phalloidin (yellow) and DAPI (blue). Bars, 10 μm. (B) EC morphology after knockdown of PHB1 (left) and incubation with PEG-catalase (right) using life imaging phase contrast microscopy. Insets show a higher magnification. Bars, 100 μm. (C) Migration of control and PHB1 knockdown cells pretreated with and without PEG-catalase was examined via migration assay in the presence and absence of VEGF. n = 4; *, P < 0.05. (D) Tube formation assays on matrigel plugs were performed and quantified using control and PHB1 knockdown cells. Representative examples of tubes are shown on the right. Error bars show the calculated standard deviation. n = 3; *, P < 0.05. Bars, 100 μm.