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. 2007 Nov 22;6:155. doi: 10.1186/1475-2875-6-155

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Copyright © 2007 Bass et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Species identification using the real-time TaqMan assay designed by Walker et al. In this example two specimens of An. gambiae s.s., (red trace) An. arabiensis, (blue trace) An. melas (green trace), An. merus (purple trace) and An. quadriannulatus (orange trace) were tested. Part A displays the cycling of FAM-labelled probe specific for An. arabiensis. Part B displays the cycling of the VIC-labelled probe designed to be specific for An. gambiae s.s. and shows the non-specific amplification of the An. melas, An. merus and An. quadriannulatus samples.