Figure 7.

User interfaces of the cloning database "SCISSORS". A: Screenshot of the data entry sheet of second step ORF PCR. B: 96-well colony PCR plate assembled by the software. The entered PCR results are automatically color-coded by the software as follows: red and grey: positive or negative colony (presence or absence of a band of expected size on the agarose gel), blue: entry clone colonies already used for plasmid preparation, yellow: colonies selected for generation of a new entry clone 96-well plate. C: User interface of entry clone plates. Clones scored positive in the control digest are automatically color-coded in green, negative clones remain white. Clicking on the plate positions opens a window to enter the sequencing result of the particular entry clone. D: Results of a working step can also be entered in a table format, as shown for the entry clone validation.