Table 2.

Success rates of ORF amplification

template type	cDNA clone				EST clone				primary cDNA				total
ORFs targeted	# total	# posa	%b	%c	# total	# posa	%b	%c	# total	# posa	%b	%c	# total	# posa	%b	%c
1. amplification	1712	1303	76	76	368	267	73	73	156	39	25	25	2236	1609	72	72
reamplificationd	409	162	40	9	101	39	39	11	117	25	21	16	627	226	36	10
amplified with alternative template	103	80	78	5	28	22	79	6	61	15	25	10	192	117	61	5
amplified with alternative 1-step primers	32	26	81	2	16	13	81	4	21	6	29	4	69	45	65	2
Sum	1712e	1571		92	368e	341		94	156e	85		55	2236f	1997		89

^a Number of ORFs with expected product size obtained by PCR.

^b Sucess rate of the respective reaction step.

^c Contribution of each step to the final PCR success rate (rounded values).

^d Reamplification with slight modifications of the PCR protocol depending on result of first amplification.

^e Sum of ORFs targeted per template type.

^f Total sum of ORFs targeted