Table 3.

Success rates of ORF cloning in dependence on the template used

		PCR		BP reaction		Entry clones
ORFs targeted	# total	# posa	%	# posb	%	# +/- stop codonc	# + stop codond	# - stop codone
cDNA clones	1712	1571	92	1477	94	1355	63	55
EST clones	368	341	93	314	92	290	9	13
Primary cDNA	156	85	54	76	89	69	1	3
Sum	2236	1997	89f	1867	93g	1714	73	71

^a Number of ORFs with expected product size obtained by PCR.

^b Number of ORFs with expected product size detected by colony-PCR of entry clones.

^c ORFs represented by at least one sequence verified entry clone with and without a stop codon.

^d,e At least one sequence verified entry clone with^(d) or without^(e) a stop codon was obtained for these ORFs.

^f,g Cumulative success rates of PCR reaction (^f) and BP cloning (^g)