Table 3.

Real time qRT-PCR primer sequences, optimum primer concentrations and amplicon sizes for all validated genes.

Array feature/Clone ID	Gene symbol	Forward primer (5'-3')	Reverse primer (5'-3')	Amplicon size (bp)	Primer conc. (nM)
Reference gene	H3F3A	CATGGCTCGTACAAAGCAGA	ACCAGGCCTGTAACGATGAG	136	100
PCR Amplicon	ACTB	AAGCCGGCCTTGCACAT	TAACTCGAGAGCCAACGTCTCC	66	900
NBFGC_BE752490	ADAM17	TCAAAGTCGTGGTGGTAGATGG	AATTAGTCTCCAAAGCGGCTCT	188	900
NBFGC_AW656779	AKT1	GAGTACTTCAGGGCCGTCAG	GGTGATCCTGGTGAAGGAGA	160	900
PCR Amplicon	BCL2	ATGACTTCTCTCGGCGCTAC	ATGACCGAGTACCTGAACCG	244	300
PCR Amplicon	CCL1	AGGCTGGATCTGCTCCCAAAT	GGTGATGTGTGCAAGTTCACCA	152	900
BOTL0100003XA07R	CD81	TTCATGTCCTGAAGCTCCCTGT	TGAAGGCATAAGGCTGCTCGT	284	300
BOTL0100013_C12	CD84	TAAGTGGTGTGTCATGGCAGGT	GGCTGGAGGCTGAATATGACTG	103	300
PCR Amplicon	CXCR3	GAAAGCAGTGTGGACATAGCCA	CGGAACTTGACACCCACAAAG	101	900
NBFGC_BF230159	EEF1G	TGGATGCTCACTTGAAGACG	ACTGGGCCATTTTCTCACAG	222	300
PCR Amplicon	GAPDH	CTCCCAACGTGTCTGTTGTG	TGAGCTTGACAAAGTGGTCG	222	300
BOTL0100013_E07	IER5	AAGACCCCCGAGACTTCG	ACACTCTTCAAGGCGGAGAG	115	300
PCR Amplicon	IFNG	TGATGGCATGTCAGACAGCA	GGCACAAGTCATATAGCCTGACAC	51	300
PCR Amplicon	IL2	CTTGCACTCGTTGCAAACG	CAAGCTCTCCAGGATGCATACA	183	300
PCR Amplicon	IL4	GCCACACGTGCTTGAACAAA	TCTCAACAGCTTGGCAAGCA	63	300
PCR amplicon	IL8	AGGTGGTGTTTGAAGCCCAT	CACAACCTTCTGCACCCACTT	123	900
PCR Amplicon	IL16	CGCGGTTTGAAGAATGGAAC	TCACAGGTCCATCAGGCAAC	51	300
PCR Amplicon	MCL1	AGGTGACTGAAAGGCCTGTCTC	CAACATGTGCCTCTTCTCCCT	244	900
BF775342 NFATC4	NFATC4	AACCACTGCCCCTCTCTGAAAC	CCTCGACCCCAGATCACAAAGA	107	300
BOTL0100003XF01R	NFKB1	ATACTGAACAATGCCTTCCGG	CACGTCAATGGCCTCAGTGTAG	135	300
BOTL0100013_G05	PHB2	GGCGGCGCGGATGT	AGGTTATATCAAGCTACGCAAGATCC	65	900
NBFGC_AW335987	PRKCB1	ATCGAGAGGGAGGTCCTCAT	GGTCTTGGTCTTCTGCTTGC	141	300
PCR Amplicon	RPL19	AATGCCCGAGAAGGTAACCTG	GGATATGTTCCATGAGGATCCG	164	100
NBFGC_AW669767	RPS6KB2	TGTGGAACTGGCCTATGCCTTC	AAGATGCCTTCTCGCTCCAGGT	105	300
PCR Amplicon	STK17B	ACAGGCCCTCTTGTAATGGCAC	AGCAAATCGGACACAAGCTCG	136	300
NBFGC_BE479784	TBK1	TGGACCAATTGACTGGAGTGGA	TGATCTGCCTCAAGGATGTTTG	105	300
Not represented	TLR2	CCATTGACAAGAAGGCCAT	AACCCTTCCTGCTGAGTCTCAT	107	900
Not represented	TLR4	CGAGAGCACCTATGATGCCTTT	ATGGCCACCCCAGGAATAAA	144	900
PCR Amplicon	TNF	TCTACCAGGGAGGAGTCTTCCA	GTCCGGCAGGTTGATCTCA	68	300

Clone IDs were obtained from the Center for Animal Functional Genomics (CAFG) website [51].