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. 2007 Nov 21;6:36. doi: 10.1186/1475-2859-6-36

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Copyright © 2007 Liang et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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SDS-PAGE analysis of crude extracts of XR, NT-XR and CT-XR. Expression was carried out in E. coli cells in LB medium containing kanamycin (50 μg ml^-1) and IPTG (1 mM). Crude extracts of different variants were adjusted to the same OD₆₀₀concentration (12.41 OD/ml) before they were prepared, and 15 μl was loaded in each lane. Lanes: 1, the insoluble fraction of NT-XR; 2, the soluble fraction of NT-XR; 3, the insoluble fraction of pET30a; 4, the soluble fraction of pET30a; 5, marker; 6, the insoluble fraction of XR; 7, the soluble fraction of XR; 8, the soluble fraction of CT-XR; 9, the insoluble fraction of CT-XR.