Figure 2.

Accumulation of hybrid TMV coat protein in tobacco leaves. Total leaf proteins extracted from infected tobacco tissues or partially purified proteins were fractionated on duplicate 12.5% SDS/12.5% PAGE. One gel was stained with Coomassie blue (Left), the other gel was used for immunoblot analysis with the monoclonal antibody to 5B19, MAb5B19 (Center), or with the polyclonal antibody specific for TMV (Right). M, Protein molecular mass. Lane 1, Extract from non-infected plant; lane 2, extract from TMV-5B19 infected plant; lane 3, extract from TMV-5B19L infected plant; lane 4, partially purified TMV-5B19; lane 5, purified TMV-5B19L; lane 6, purified w.t. TMV.