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. 2008 Jan 25;4(1):e17. doi: 10.1371/journal.pgen.0040017

Figure 1. Hyperactivation of SIN Signaling in nuc2-663 Mutant.

Figure 1

(A) nuc2-663 cells were stained with DAPI and aniline blue to visualize nuclei and septum material, respectively. The left panel shows nuc2-663 cells at permissive temperature. The right panel shows nuc2-663 cells at restrictive temperature.

(B) Quantification of multiseptated cells in the nuc2-663 mutant.

(C) Wild-type and nuc2-663 cells expressing GFP tagged versions of Cdc7p, Sid1p, and Sid2p were grown at 36 °C for 4 h and were visualized using fluorescence microscopy. Arrowheads indicate mitotic cells with Sid1p at both SPBs. Arrows indicate cells in which Sid2p-GFP signal persisted at the division site after completion of cytokinesis. Scale bar, 5 μm.