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. 2008 Jan 25;4(1):e17. doi: 10.1371/journal.pgen.0040017

Figure 5. Overexpression of Nuc2p Does Not Affect the Steady-State Levels of Cdc7p-3HA and Sid2p-13Myc, but Disrupts the Binding of Spg1p-GFP and Cdc7p-3HA.

Figure 5

(A) Protein levels of Cdc7p-3HA and Sid2p-13Myc in nmt1-nuc2 cells grown in medium with or without thiamine. Tubulin serves as the loading control.

(B) Lysates from cells expressing Cdc7p-3HA and Spg1p-GFP in the presence or absence of Nuc2p overexpression were immunoprecipitated with GFP antibodies and immunoblotted with antibodies against the HA epitope. Lysates from a strain without Spg1p-GFP was included as the control. Tubulin serves as the loading control. Asterisk indicates heavy chain of IgG.