Figure 6. Inactivation of Cdc16p Function Promotes Localization of Cdc7p to SPBs and Allows Septation in Cells Overexpressing Nuc2p.
(A) Diagram schematically illustrates the experimental design. Nuc2p was induced for 24 h at 26 °C, and the culture was shifted to 36 °C to inactivate Cdc16p and examined after 3 h of incubation.
(B) The localization of Cdc7p-GFP in cdc16-116 cells overexpressing Nuc2p was visualized by fluorescence microscopy at the permissive or restrictive temperatures.
(C) Septum assembly is restored upon inactivation of Cdc16p in cells overexpressing Nuc2p. DAPI and aniline blue staining of formaldehyde fixed cells is shown.
(D) Quantification of septated cells versus non-septated cells in nmt1-nuc2 and nmt1-nuc2 cdc16-116. Note that only cells with four nuclei were counted. Scale bar, 5 μm.