Fig 5.

Dex enhances Mcl-1L protein stability in neutrophils, but not in eosinophils. Neutrophils (A and C) and eosinophils (B and D) were cultured with CHX (10 μg/ml) to eliminate de novo protein synthesis. Cells were either cultured in the absence or presence of Dex (20 μM) to determine the effect of Dex on the stability of existing Mcl-1L protein. Neutrophils and eosinophils were analyzed by Western blot, as in figure 2, at 0, 2, 4, and 6 h to determine how much of the initial Mcl-1L was still present. Densitometry of western blots is graphed on a log scale as a percent of the 0 h control value for neutrophils (C) and eosinophils (D) treated with CHX alone (closed circles) or treated with CHX and Dex (open circles). The solid lines represent the best fit exponential decay for CHX-treated samples and the dashed lines represent the best fit exponential decay for CHX and Dex-treated samples. * denotes p<0.05 for Dex-treated cells versus control cells at each time point.