Fig 6.

Mcl-1 antisense oligos induce neutrophil apoptosis and inhibit the anti-apoptotic effect of Dex. Neutrophils were cultured in media alone (C), or with the addition of Mcl-1 antisense oligos (AS), Dex, Dex plus Mcl-1 antisense (Dex + AS), Dex plus Mcl-1 sense (Dex + S), or Mcl-1 sense oligos (S), for 12 h. A) Neutrophils were cytocentrifuged onto slides, and stained with Diff Quik in order to determine the percentage of cells demonstrating apoptotic morphology. * denotes p< 0.02 for Dex versus control and for antisense versus control, ** denotes p< 0.02 for Dex + AS versus Dex alone, n=6. B) Western blot of neutrophil protein confirmed that Mcl-1 antisense oligos (AS) knocked down Mcl-1 protein after 6 h of treatment relative to media alone (M), sense oligos (S), vehicle control (C), Dex, or proteasome protection by MG-132 (MG) (representative experiment shown, n=2).