Figure 3.

No change in markers for enterocyte differentiation or cell lineages due to ectopic Cdx1 expression. (A) Immunohistochemistry with anti-sucrase isomaltase antibody shows no change in pattern of sucrase isomaltase gene expression in the small intestine brush border between 7-month-old wild-type and villin-Cdx1 transgenic mice. No difference in activity of brush border enzyme alkaline phosphatase noted in 7-month-old wild-type and transgenic mice. (C) Seven-month-old wild-type and villin-Cdx1 transgenic mice show no difference in the distribution of immunohistochemical staining for an enteroendocrine cell marker (chromagranin-A) or a paneth cell marker (lysozyme). (B) Similarly, staining with Alcian blue, a marker for goblet cells in the ileum of the small intestine and colon, was unchanged by ectopic Cdx1 expression in wild-type and transgenic small intestine.