Figure 4.

Ectopic expression of Cdx1 reduces Cdx2 expression in the small intestine villi and colon surface epithelium. Immunohistochemistry with either Cdx1 or Cdx2 antibodies on adjacent sections from 9-month-old nontransgenic and Villin-Cdx1 littermates. (A) Small intestine of nontransgenic mice with normal Cdx1 and Cdx2 expressions. Arrow points to reduced Cdx2 staining. (B) Cdx1 and Cdx2 expression pattern in the colon of transgenic and nontransgenic mice. Arrowhead points to reduced Cdx2 staining. (C) Real-time RT-PCR quantification of Cdx2 mRNA levels in the small intestine of transgenic and nontransgenic littermates. (D) Endogenous Cdx1 and total mRNA levels in the small intestine determined by quantitative RT-PCR. WT, wild-type; Tg, transgenic; NS, nonsignificant differences.