Table 1.

Polyp Formation in Transgenic and Control Mice with AOM and DSS Treatment.

	Villin-Cdx1	Nontransgenic Littermate
AOM and two rounds of 3% DSS	20 adenomas and 1 carcinoma in six mice (100% mice with polyps)	50 adenomas in six mice (100% mice with polyps)
AOM and two rounds of water	0 adenomas	0 adenomas
Vehicle and two rounds of 3% DSS	0 adenomas	0 adenomas
AOM and three rounds of 3% DSS	64 Adenomas in 11 mice (100% mice with polyps)	88 adenomas in nine mice (100% mice with polyps)
AOM and three rounds of water	0 adenomas	0 adenomas
Vehicle and three rounds of 3% DSS	0 adenomas	0 adenomas

Six-week-old villin-Cdx1 mice and nontransgenic littermate controls were given intraperitoneal injections of AOM (10 mg/kg) or vehicle. One week later, they received 3% DSS in their drinking water for 7 days. Mice received one or two additional rounds of 3% DSS for 7 days. Fourteen weeks after the initial AOM injection, mice were sacrificed; the number of colonic polyps was scored and adenomas were confirmed histologically.