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. 1999 Jul 6;96(14):7791–7796. doi: 10.1073/pnas.96.14.7791

Figure 4.

Figure 4

Recruitment of hCycT1 to the HIV-1 LTR promoter enhances transcriptional processivity. (a) Schematic representation of the pHIV/SLIIB/CAT reporter plasmid indicating the positions of the PV UTR and Dist probes used to measure the levels of promoter proximal (T1), intermediate (T2), and distal (Dist) RNA. (b) Ribonuclease protection assay performed by using total RNA extracted from 293T cells transfected with pHIV/SLIIB/CAT and a plasmid expressing Rev (lane 1), Tat-Rev (lane 2), hCycT1-Rev (lane 3), hCycT1(X5)-Rev (lane 4), or hCycT1(K26)-Rev (lane 5). A mock protection assay (lane 6) used RNA from untransfected 293T cells.