Figure 5.

Comparison of the pH-dependence of tyrosine nitration of GS and BSA in the presence of physiological concentrations of CO₂. Reaction mixtures (1.0 ml) containing 3.0 mM PN and 1.0 mg of BSA (A) or 1.0 mg of GS (B) and bicarbonate concentrations needed to obtain the indicated pH values were prepared and incubated as described in Fig. 4. Then, the levels of nitrotyrosine were determined as described in Materials and Methods.