Table 1.
Bait | Transformants tested (×10−6) | 3ATR β-Gal+ clones | Clone sequenced | Transcription complex subunit* |
---|---|---|---|---|
C160 | 3.0 | 0 | 0 | None |
C128 | 6.5 | 0 | 0 | None |
C82 | 24.0 | 72 | 39 | C34 (1, 1) |
C53 | 13.6 | 55 | 38 | TFC4 (1, 1); C37 (15, 4) |
AC40 | 50.4 | 235† | 109 | AC19 (19, 4); A135 (4, 2) |
ABC27 | 7.2 | 207 | 118 | A190 (12, 10); B220 (2, 1); C160 (7, 6) |
C25 | 15.0 | 111 | 71 | None |
ABC23 | 5.5 | 0 | 0 | None |
AC19 | 38.3 | 450† | 153 | AC40 (6, 5); A135 (3, 2); B44 (2, 2) |
ABC14.5 | 12.0 | 31 | 29 | B220 (1, 1); C160 (6, 4) |
C11 | 10.6 | 101 | 83 | C128 (2, 2) |
ABC10α | 7.3 | 196 | 122 | A135 (1, 1) |
ABC10β | 113.2 | 276 | 209 | B220 (1, 1); B150 (2, 1) |
TFIIIB70 | 9.2 | 96 | 82 | TFC4 (2, 2) |
A12.2 | 11.0 | 105 | 82 | A135 (1, 1) |
B12.5 | 19.3 | 302 | 185 | B44 (27, 18) |
The numbers between the parentheses represent the total number of selected clones and the number of independent clones, respectively.
Only 320 of the 968 3ATR clones selected in the AC40 screen and 632 of the 2,136 3ATR clones selected in the AC29 screen were tested for β-galactosidase production.