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. 2007 Aug 23;38(2):153–160. doi: 10.1165/rcmb.2007-0173OC

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Figure 4. — Airway epithelial cells release EGF and HB-EGF in response to mechanical injury. Total protein lysates and CM were collected from injured monolayers of 1HAEo⁻ cells at indicated times. (A) Release of EGF into CM was measured by ELISA. (B) Synthesis and (C) release of HB-EGF after mechanical injury were examined by Western blotting and ELISA. All membranes were stained with Ponceau S to confirm loading. Synthesis of HB-EGF after injury was studied in both serum-free medium (SFM) and in the presence of 10% FCS (B), while release was examined only in serum-free conditions (C). *P < 0.05, ^‡P < 0.01.

Figure 4. — Airway epithelial cells release EGF and HB-EGF in response to mechanical injury. Total protein lysates and CM were collected from injured monolayers of 1HAEo⁻ cells at indicated times. (A) Release of EGF into CM was measured by ELISA. (B) Synthesis and (C) release of HB-EGF after mechanical injury were examined by Western blotting and ELISA. All membranes were stained with Ponceau S to confirm loading. Synthesis of HB-EGF after injury was studied in both serum-free medium (SFM) and in the presence of 10% FCS (B), while release was examined only in serum-free conditions (C). *P < 0.05, ^‡P < 0.01.

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