Figure 6.

IL-13 enhances production and release of HB-EGF in a culture model of airway epithelium. Confluent monolayers of 1HAEo⁻ cells were treated with IL-13 (10 ng/ml) and total protein lysates and CM were collected. (A) Synthesis and (B) release of HB-EGF and release of EGF were examined by western blotting and ELISA. In each experiment, monolayers with no treatment were considered as control. Data in B are expressed as fold increase relative to the control monolayers with no treatment (gray and black bars). IL-13 induced (A) synthesis and (B) release of HB-EGF from 1HAEo⁻ cells in a time-dependent manner. IL-13 had no effect on release of EGF by 1HAEo⁻ cells (B).